SECURITIES AND EXCHANGE COMMISSION
                             WASHINGTON, D.C. 20549


                                  SCHEDULE 13G


                   Under the Securities Exchange Act of 1934

                               (Amendment No. 4)*

                            AMES NATIONAL CORPORATION
                                (Name of Issuer)


                                  COMMON STOCK
                         (Title of Class of Securities)


                                    031001100
                                 (CUSIP Number)


                                December 31, 2005
            (Date of Event Which Requires Filing of this Statement)



Check the  appropriate box to designate the rule pursuant to which this Schedule
is filed:

     [_] Rule 13d-1(b)
     [X] Rule 13d-1(c)
     [_] Rule 13d-1(d)

   * The  remainder  of this  cover  page  shall be filled  out for a  reporting
     person's  initial  filing on this form with respect to the subject class of
     securities,  and for any subsequent amendment containing  information which
     would alter disclosure provided in a prior cover page.

The information required on the remainder of this cover page shall not be deemed
to be "filed" for the purpose of Section 18 of the  Securities  Exchange  Act of
1934 ("Act") or otherwise  subject to the liabilities of that section of the Act
but  shall be  subject  to all other  provisions  of the Act  (however,  see the
Notes).

                                       1


1.   Name of Reporting Person

     Robert W. Stafford

     I.R.S. Identification No. of Above Person (entities only)

     Not Applicable

2.   Check the appropriate box if a member of a group

     (a) ______
     (b) ___X__

3.   SEC Use Only

4.   Citizenship or Place of Organization

     Iowa


NUMBER OF SHARES BENEFICIALLY OWNED BY EACH REPORTING PERSON WITH:


5.   Sole Voting Power

     507,584

6.   Shared Voting Power

     430,027

7.   Sole Dispositive Power

     507,584

8.   Shared Dispositive Power

     430,027


9.   Aggregate Amount Beneficially Owned by Each Reporting Person

     937,611

10.  Check box if the aggregate amount in Row (9) excludes certain shares


11.  Percent of Class Represented by Amount in Row 9

     9.95%


12.  Type of Reporting Person

     IN

                                       2


1.   Name of Reporting Person

     Charlotte H. Stafford

     I.R.S. Identification No. of Above Person (entities only)

     Not Applicable

2.   Check the appropriate box if a member of a group

     (a) ________
     (b) ____X___

3.   SEC Use Only

4.   Citizenship or Place of Organization

     Texas


NUMBER OF SHARES BENEFICIALLY OWNED BY EACH REPORTING PERSON WITH:


5.   Sole Voting Power

     34,140

6.   Shared Voting Power

     430,027

7.   Sole Dispositive Power

     34,140

8.   Shared Dispositive Power

     430,027

9.   Aggregate Amount Beneficially Owned by Each Reporting Person

     464,167

10.  Check box if the aggregate amount in Row (9) excludes certain shares

11.  Percent of Class Represented by Amount in Row 9

     4.9%

12.  Type of Reporting Person

     IN

                                       3


ITEM 1(a). NAME OF ISSUER.

           Ames National Corporation

ITEM 1(b). ADDRESS OF ISSUER'S PRINCIPAL EXECUTIVE OFFICES.

           P.O. Box 846 Ames, IA 50010

ITEM 2(a). NAME OF PERSON FILING.

           This filing is made on behalf of Robert W.  Stafford and Charlotte H.
           Stafford. Mr. Stafford and Ms. Stafford each disclaim membership in a
           group.  Mr.  Stafford  disclaims  beneficial  ownership of the 34,140
           shares over which Ms. Stafford has sole voting and dispositive power.
           Ms.  Stafford  disclaims  beneficial  ownership of the 507,584 shares
           over which Mr.  Stafford has sole voting and dispositive  power.  Mr.
           Stafford  and  Ms.  Stafford  are  co-trustees  of  two  trusts  with
           aggregate  holdings of 430,027 shares over which Mr. Stafford and Ms.
           Stafford  have shared voting and  dispositive  power.  Mr.  Stafford,
           however,  disclaims any pecuniary  interest in any of the shares held
           by the trusts.

ITEM 2(b). ADDRESS OF THE PRINCIPAL OFFICES OR RESIDENCE:

           Robert W.  Stafford's  business  address is P.O. Box 846, Ames,  Iowa
           50010 and  Charlotte H.  Stafford's  residence  address is 9701 Meyer
           Forest Drive, Apt. 12202, Houston, Texas 77096-4324.

ITEM 2(c). CITIZENSHIP.

           Robert W. Stafford - Iowa Charlotte H. Stafford - Texas

ITEM 2(d). TITLE OF CLASS OF SECURITIES.

           Common Stock.


ITEM 2(e). CUSIP NUMBER.

           031001100


ITEM 3.    IF THE STATEMENT IS BEING FILED PURSUANT TO SECTION 240.13d-1(b),
           CHECK WHETHER THE PERSON FILING IS A:

           Not Applicable

ITEM 4.    OWNERSHIP.

           As of  December  31,  2005,  Robert  W.  Stafford  and  Charlotte  H.
           Stafford,  collectively,  beneficially  owned an aggregate of 971,751
           shares  of the  common  stock  of Ames  National  Corporation,  which
           represented approximately 10.3 percent of the outstanding shares. Mr.
           Stafford and Ms.  Stafford each disclaim  membership in a group,  and
           their  individual  share  ownership and  percentages  and information
           regarding the manner in which the shares are  beneficially  owned are
           set forth in their  respective  cover pages.  Mr. Stafford  disclaims
           beneficial ownership of the 34,140 shares over which Ms. Stafford has
           sole voting and dispositive power. Ms. Stafford disclaims  beneficial
           ownership  of the  507,584  shares over which Mr.  Stafford  has sole
           voting and  dispositive  power.  Mr.  Stafford  and Ms.  Stafford are
           co-trustees of two trusts with  aggregate  holdings of 430,027 shares
           over which Mr.  Stafford  and Ms.  Stafford  have  shared  voting and
           dispositive  power.  Mr. Stafford,  however,  disclaims any pecuniary
           interest in any of the shares held by the trust.

ITEM 5.    OWNERSHIP OF FIVE PERCENT OR LESS OF A CLASS.

           Not Applicable


ITEM 6.    OWNERSHIP OF MORE THAN FIVE PERCENT ON BEHALF OF ANOTHER PERSON.

           Not Applicable


ITEM 7.    IDENTIFICATION  AND CLASSIFICATION OF THE SUBSIDIARY WHICH ACQUIRED
           THE SECURITY BEING REPORTED ON BY THE PARENT HOLDING COMPANY.

           Not Applicable


ITEM 8.    IDENTIFICATION AND CLASSIFICATION OF MEMBERS OF THE GROUP.

           Not Applicable

                                       4


ITEM 9.    NOTICE OF DISSOLUTION OF A GROUP.

           Not Applicable


ITEM 10.   CERTIFICATION.

           By signing  below,  I certify  that,  to the best of my knowledge and
           belief,  the  securities  referred  to  above  were  acquired  in the
           ordinary  course of business and were not acquired for the purpose of
           and do not have the effect of changing or influencing  the control of
           the issuer of such  securities  and were not  acquired in  connection
           with or as a participant  in any  transaction  having such purpose or
           effect.


                                       5


                                    SIGNATURE

After  reasonable  inquiry  and to the best of its  knowledge  and  belief,  the
undersigned  certifies that the information set forth in this statement is true,
complete and correct.


                                    BY:  /s/ Robert W. Stafford
                                        ----------------------------------------

                                    Name      Robert W. Stafford


                                    BY:  /s/ Charlotte H. Stafford
                                         ---------------------------------------

                                    Name      Charlotte H. Stafford


Date:    February 14, 2006
         -----------------



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                                                                       EXHIBIT A

                             JOINT FILING AGREEMENT


Robert W.  Stafford  and  Charlotte H.  Stafford  agree that the Schedule 13G to
which this Joint Filing  Agreement is attached,  relating to the Common Stock of
Ames National Corporation, is filed on behalf of each of them.




                                    BY: /s/ Robert W. Stafford
                                       -----------------------------------------

                                    Name      Robert W. Stafford

                                    BY: /s/ Charlotte H. Stafford
                                       -----------------------------------------

                                    Name      Charlotte H. Stafford


Date:    February 14, 2006
         -----------------


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